phosphatase inhibitor buffer Search Results


lysis buffer supplemented protease inhibitor cocktail  (Nacalai)
90
Nacalai lysis buffer supplemented protease inhibitor cocktail
Lysis Buffer Supplemented Protease Inhibitor Cocktail, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein lysis buffer supplemented protease phosphatase inhibitor cocktail  (Beyotime)
90
Beyotime protein lysis buffer supplemented protease phosphatase inhibitor cocktail
Protein Lysis Buffer Supplemented Protease Phosphatase Inhibitor Cocktail, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein lysis buffer supplemented protease phosphatase inhibitor cocktail - by Bioz Stars, 2026-03
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whole cell lysis assay kgp 250-2100  (Keygen Biotech)
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Keygen Biotech whole cell lysis assay kgp 250-2100
Whole Cell Lysis Assay Kgp 250 2100, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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whole cell lysis assay kgp 250-2100 - by Bioz Stars, 2026-03
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ripa buffer containing protease and phosphatase inhibitors  (GenDEPOT)
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GenDEPOT ripa buffer containing protease and phosphatase inhibitors
Ripa Buffer Containing Protease And Phosphatase Inhibitors, supplied by GenDEPOT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ripa buffer containing protease and phosphatase inhibitors - by Bioz Stars, 2026-03
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tris lysis buffer containing phosphatase inhibitors i and ii and protease inhibitor  (Meso Scale Diagnostics LLC)
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Meso Scale Diagnostics LLC tris lysis buffer containing phosphatase inhibitors i and ii and protease inhibitor
Tris Lysis Buffer Containing Phosphatase Inhibitors I And Ii And Protease Inhibitor, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
tris lysis buffer containing phosphatase inhibitors i and ii and protease inhibitor - by Bioz Stars, 2026-03
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lysis buffer supplement with protease and phosphatase inhibitor  (Beyotime)
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Beyotime lysis buffer supplement with protease and phosphatase inhibitor
Lysis Buffer Supplement With Protease And Phosphatase Inhibitor, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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cell lysis buffer radioimmunoprecipitation lysis buffer with protease and phosphatase inhibitor cocktail  (Beyotime)
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Beyotime cell lysis buffer radioimmunoprecipitation lysis buffer with protease and phosphatase inhibitor cocktail
Cell Lysis Buffer Radioimmunoprecipitation Lysis Buffer With Protease And Phosphatase Inhibitor Cocktail, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoprecipitation buffer  (Bimake Inc)
90
Bimake Inc immunoprecipitation buffer
METTL3-dependent m 6 A modification regulates DGCR8 processing of miR-181d-5p. (A) <t>Co-immunoprecipitation</t> analysis of DGCR8. (B) Immunoprecipitation of DGCR8 in METTL3-overexpressing cells. (C) Expression of miRNAs in exosomes derived from METTL3-overexpressing or METTL3-silencing CAFs. (D) Expression of pri-miR-181d, pre-miR-181d and miR-181d-5p and (E) the levels of pri-miR-181d-5p m 6 A in METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. (F) Analysis of pri-miRNA binding to DGCR8. (G) Analysis of pri-miRNAs m 6 A modification. (H and I) miR-181d-5p levels in CRC cells treated with exosomes derived from METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. All experiments were performed in triplicate. * P<0.05, ** P<0.01, *** P<0.001. METTL, methyltransferase like; m 6 A, RNA N6-methyladenosine; DGCR8, DiGeorge Syndrome Critical Region 8; miR/miRNA, microRNA; CAFs, cancer-associated fibroblasts; pri, primary; pre, precursor; sh, short hairpin; NC, negative control; CAFs-exo, primary CAFs.
Immunoprecipitation Buffer, supplied by Bimake Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunoprecipitation buffer/product/Bimake Inc
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immunoprecipitation buffer - by Bioz Stars, 2026-03
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ripa buffer supplemented with protease/phosphatase inhibitors b15001  (Biomark Inc)
90
Biomark Inc ripa buffer supplemented with protease/phosphatase inhibitors b15001
METTL3-dependent m 6 A modification regulates DGCR8 processing of miR-181d-5p. (A) <t>Co-immunoprecipitation</t> analysis of DGCR8. (B) Immunoprecipitation of DGCR8 in METTL3-overexpressing cells. (C) Expression of miRNAs in exosomes derived from METTL3-overexpressing or METTL3-silencing CAFs. (D) Expression of pri-miR-181d, pre-miR-181d and miR-181d-5p and (E) the levels of pri-miR-181d-5p m 6 A in METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. (F) Analysis of pri-miRNA binding to DGCR8. (G) Analysis of pri-miRNAs m 6 A modification. (H and I) miR-181d-5p levels in CRC cells treated with exosomes derived from METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. All experiments were performed in triplicate. * P<0.05, ** P<0.01, *** P<0.001. METTL, methyltransferase like; m 6 A, RNA N6-methyladenosine; DGCR8, DiGeorge Syndrome Critical Region 8; miR/miRNA, microRNA; CAFs, cancer-associated fibroblasts; pri, primary; pre, precursor; sh, short hairpin; NC, negative control; CAFs-exo, primary CAFs.
Ripa Buffer Supplemented With Protease/Phosphatase Inhibitors B15001, supplied by Biomark Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ripa buffer supplemented with protease/phosphatase inhibitors b15001/product/Biomark Inc
Average 90 stars, based on 1 article reviews
ripa buffer supplemented with protease/phosphatase inhibitors b15001 - by Bioz Stars, 2026-03
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ripa buffer with beyotime protease and phosphatase inhibitors  (Beyotime)
90
Beyotime ripa buffer with beyotime protease and phosphatase inhibitors
METTL3-dependent m 6 A modification regulates DGCR8 processing of miR-181d-5p. (A) <t>Co-immunoprecipitation</t> analysis of DGCR8. (B) Immunoprecipitation of DGCR8 in METTL3-overexpressing cells. (C) Expression of miRNAs in exosomes derived from METTL3-overexpressing or METTL3-silencing CAFs. (D) Expression of pri-miR-181d, pre-miR-181d and miR-181d-5p and (E) the levels of pri-miR-181d-5p m 6 A in METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. (F) Analysis of pri-miRNA binding to DGCR8. (G) Analysis of pri-miRNAs m 6 A modification. (H and I) miR-181d-5p levels in CRC cells treated with exosomes derived from METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. All experiments were performed in triplicate. * P<0.05, ** P<0.01, *** P<0.001. METTL, methyltransferase like; m 6 A, RNA N6-methyladenosine; DGCR8, DiGeorge Syndrome Critical Region 8; miR/miRNA, microRNA; CAFs, cancer-associated fibroblasts; pri, primary; pre, precursor; sh, short hairpin; NC, negative control; CAFs-exo, primary CAFs.
Ripa Buffer With Beyotime Protease And Phosphatase Inhibitors, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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lysis buffer supplemented protease phosphatase inhibitors  (Beyotime)
90
Beyotime lysis buffer supplemented protease phosphatase inhibitors
METTL3-dependent m 6 A modification regulates DGCR8 processing of miR-181d-5p. (A) <t>Co-immunoprecipitation</t> analysis of DGCR8. (B) Immunoprecipitation of DGCR8 in METTL3-overexpressing cells. (C) Expression of miRNAs in exosomes derived from METTL3-overexpressing or METTL3-silencing CAFs. (D) Expression of pri-miR-181d, pre-miR-181d and miR-181d-5p and (E) the levels of pri-miR-181d-5p m 6 A in METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. (F) Analysis of pri-miRNA binding to DGCR8. (G) Analysis of pri-miRNAs m 6 A modification. (H and I) miR-181d-5p levels in CRC cells treated with exosomes derived from METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. All experiments were performed in triplicate. * P<0.05, ** P<0.01, *** P<0.001. METTL, methyltransferase like; m 6 A, RNA N6-methyladenosine; DGCR8, DiGeorge Syndrome Critical Region 8; miR/miRNA, microRNA; CAFs, cancer-associated fibroblasts; pri, primary; pre, precursor; sh, short hairpin; NC, negative control; CAFs-exo, primary CAFs.
Lysis Buffer Supplemented Protease Phosphatase Inhibitors, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer supplemented protease phosphatase inhibitors/product/Beyotime
Average 90 stars, based on 1 article reviews
lysis buffer supplemented protease phosphatase inhibitors - by Bioz Stars, 2026-03
90/100 stars
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lysis buffer containing 1% protease inhibitor cocktail  (Beijing CWBio)
90
Beijing CWBio lysis buffer containing 1% protease inhibitor cocktail
METTL3-dependent m 6 A modification regulates DGCR8 processing of miR-181d-5p. (A) <t>Co-immunoprecipitation</t> analysis of DGCR8. (B) Immunoprecipitation of DGCR8 in METTL3-overexpressing cells. (C) Expression of miRNAs in exosomes derived from METTL3-overexpressing or METTL3-silencing CAFs. (D) Expression of pri-miR-181d, pre-miR-181d and miR-181d-5p and (E) the levels of pri-miR-181d-5p m 6 A in METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. (F) Analysis of pri-miRNA binding to DGCR8. (G) Analysis of pri-miRNAs m 6 A modification. (H and I) miR-181d-5p levels in CRC cells treated with exosomes derived from METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. All experiments were performed in triplicate. * P<0.05, ** P<0.01, *** P<0.001. METTL, methyltransferase like; m 6 A, RNA N6-methyladenosine; DGCR8, DiGeorge Syndrome Critical Region 8; miR/miRNA, microRNA; CAFs, cancer-associated fibroblasts; pri, primary; pre, precursor; sh, short hairpin; NC, negative control; CAFs-exo, primary CAFs.
Lysis Buffer Containing 1% Protease Inhibitor Cocktail, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
lysis buffer containing 1% protease inhibitor cocktail - by Bioz Stars, 2026-03
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Image Search Results


METTL3-dependent m 6 A modification regulates DGCR8 processing of miR-181d-5p. (A) Co-immunoprecipitation analysis of DGCR8. (B) Immunoprecipitation of DGCR8 in METTL3-overexpressing cells. (C) Expression of miRNAs in exosomes derived from METTL3-overexpressing or METTL3-silencing CAFs. (D) Expression of pri-miR-181d, pre-miR-181d and miR-181d-5p and (E) the levels of pri-miR-181d-5p m 6 A in METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. (F) Analysis of pri-miRNA binding to DGCR8. (G) Analysis of pri-miRNAs m 6 A modification. (H and I) miR-181d-5p levels in CRC cells treated with exosomes derived from METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. All experiments were performed in triplicate. * P<0.05, ** P<0.01, *** P<0.001. METTL, methyltransferase like; m 6 A, RNA N6-methyladenosine; DGCR8, DiGeorge Syndrome Critical Region 8; miR/miRNA, microRNA; CAFs, cancer-associated fibroblasts; pri, primary; pre, precursor; sh, short hairpin; NC, negative control; CAFs-exo, primary CAFs.

Journal: International Journal of Oncology

Article Title: N6-methyladenosine upregulates miR-181d-5p in exosomes derived from cancer-associated fibroblasts to inhibit 5-FU sensitivity by targeting NCALD in colorectal cancer

doi: 10.3892/ijo.2022.5304

Figure Lengend Snippet: METTL3-dependent m 6 A modification regulates DGCR8 processing of miR-181d-5p. (A) Co-immunoprecipitation analysis of DGCR8. (B) Immunoprecipitation of DGCR8 in METTL3-overexpressing cells. (C) Expression of miRNAs in exosomes derived from METTL3-overexpressing or METTL3-silencing CAFs. (D) Expression of pri-miR-181d, pre-miR-181d and miR-181d-5p and (E) the levels of pri-miR-181d-5p m 6 A in METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. (F) Analysis of pri-miRNA binding to DGCR8. (G) Analysis of pri-miRNAs m 6 A modification. (H and I) miR-181d-5p levels in CRC cells treated with exosomes derived from METTL3-overexpressing or METTL3-silencing lentivirus-transduced CAFs. All experiments were performed in triplicate. * P<0.05, ** P<0.01, *** P<0.001. METTL, methyltransferase like; m 6 A, RNA N6-methyladenosine; DGCR8, DiGeorge Syndrome Critical Region 8; miR/miRNA, microRNA; CAFs, cancer-associated fibroblasts; pri, primary; pre, precursor; sh, short hairpin; NC, negative control; CAFs-exo, primary CAFs.

Article Snippet: Cells were lysed using immunoprecipitation buffer (containing 1 mM DTT, 100 mmol/l NaCl and 1 mM MgCl 2 ) and protease inhibitor cocktails ( Bimake.com ).

Techniques: Modification, Immunoprecipitation, Expressing, Derivative Assay, Binding Assay, Negative Control